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high-throughput snp genotyping with microarray technology  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high-throughput snp genotyping with microarray technology
    High Throughput Snp Genotyping With Microarray Technology, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+throughput+microarray/pmc11675768-136-6-0
    Average 90 stars, based on 1 article reviews
    high-throughput snp genotyping with microarray technology - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Discovery of a novel highly specific, fully human PSCA antibody and its application as an antibody-drug conjugate in prostate cancer
    Article Snippet: .. The PSCA expression on PC-3 and Du-145 PSCA transgenic cell lines were confirmed by PE-conjugated anti-Flag tag antibody and the murine PSCA antibody 7F5 followed by PE-conjugated anti-mouse IgG antibody (ThermoFisher, CAT#P-852). ..

    Transgenic Assay:

    Article Title: Discovery of a novel highly specific, fully human PSCA antibody and its application as an antibody-drug conjugate in prostate cancer
    Article Snippet: .. The PSCA expression on PC-3 and Du-145 PSCA transgenic cell lines were confirmed by PE-conjugated anti-Flag tag antibody and the murine PSCA antibody 7F5 followed by PE-conjugated anti-mouse IgG antibody (ThermoFisher, CAT#P-852). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of targetable vulnerabilities of PLK1-overexpressing cancers by synthetic dosage lethality
    Article Snippet: cDNA templates for droplet digital PCR were prepared with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The 20 μL quantitative polymerase chain reaction was performed using 10 μL ddPCRTM Supermix for Probes (Bio-Rad), 1 μL of target gene probe ( IGF2BP2 : FAM-MGB, Hs00538954_g1, PLK1 : FAM-MGB, Hs00983227_m1, Thermo Scientific), 1 μL of reference probe ( GAPDH : VIC-MGB, Hs02786624_g1, Thermo Scientific), and 50 ng of cDNA template. .. Droplets were generated from a QX100 ddPCR Droplet generator (Bio-Rad Laboratories).

    Article Title: Identification of targetable vulnerabilities of PLK1-overexpressing cancers by synthetic dosage lethality
    Article Snippet: cDNA templates for droplet digital PCR were prepared using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The 20 μL quantitative polymerase chain reaction was performed using 10 μL ddPCR Supermix for Probes (Bio-Rad), 1 μL of target gene probe ( IGF2BP2 : FAM-MGB, Hs00538954_g1, PLK1 : FAM-MGB, Hs00983227_m1, Thermo Scientific), 1 μL of reference probe ( GAPDH : VIC-MGB, Hs02786624_g1, Thermo Scientific), and 50 ng of cDNA template. .. Droplets were generated using a QX100 ddPCR droplet generator (Bio-Rad Laboratories).

    Software:

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody–drug conjugate–resistant HER2-overexpressing breast cancer
    Article Snippet: .. Transcriptome Analysis Console (TAC, Affymetrix, Inc) software used for the Affymetrix Clariom D Human microarray database to compare differential gene splicing between HER2-ADC-resistant cells and their parent cells. ..

    Microarray:

    Article Title: The DNA repair pathway as a therapeutic target to synergize with trastuzumab deruxtecan in HER2-targeted antibody–drug conjugate–resistant HER2-overexpressing breast cancer
    Article Snippet: .. Transcriptome Analysis Console (TAC, Affymetrix, Inc) software used for the Affymetrix Clariom D Human microarray database to compare differential gene splicing between HER2-ADC-resistant cells and their parent cells. ..

    other:

    Article Title: Mature microRNA-binding protein QKI suppresses extracellular microRNA let-7b release
    Article Snippet: The chips were washed five more times with RNase-free PBS buffer for 10 min. For detection of all possible proteins, the chips were then incubated with rabbit anti-GST antibody (71-7500, Invitrogen) at 1:5000 for 1 h at room temperature, washed three times for 15 min in RNase-free PBS buffer, and incubated with 1:1000 Cy3 goat anti-rabbit IgG antibody (A10520, Invitrogen) in RNase-free PBS buffer for 20 min at room temperature.

    Article Title: Identification of targetable vulnerabilities of PLK1-overexpressing cancers by synthetic dosage lethality
    Article Snippet: GAPDH probe , Thermo Scientific , VIC-MGB, Hs02786624_g1.



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    Image Search Results


    Flowchart of the study Early-LUAD, early-stage lung adenocarcinoma; BLD, benign lung disease; NHC, normal healthy control; HuProt TM , Human Proteome Microarray; ELISA, enzyme-linked immunosorbent assay.

    Journal: Genomics, Proteomics & Bioinformatics

    Article Title: A Novel IgG–IgM Autoantibody Panel Enhances Detection of Early-stage Lung Adenocarcinoma from Benign Nodules

    doi: 10.1093/gpbjnl/qzae085

    Figure Lengend Snippet: Flowchart of the study Early-LUAD, early-stage lung adenocarcinoma; BLD, benign lung disease; NHC, normal healthy control; HuProt TM , Human Proteome Microarray; ELISA, enzyme-linked immunosorbent assay.

    Article Snippet: The high-throughput protein microarray HuProt TM v4.0 was obtained from CDI Laboratories (Catalog No. CDIHP-004, Baltimore, MD).

    Techniques: Control, Microarray, Enzyme-linked Immunosorbent Assay

    Profiling of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control in the HuProt TM screening The heatmap displays a distinct distribution of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control, with generally higher levels of IgM observed in Early-LUAD. All values are normalized. The plots on the top display the data on age, sex, smoking, and alcohol consumption for each group, while the bar chart on the right side shows the sensitivity of each autoantibody. Control indicates the BLD+NHC group.

    Journal: Genomics, Proteomics & Bioinformatics

    Article Title: A Novel IgG–IgM Autoantibody Panel Enhances Detection of Early-stage Lung Adenocarcinoma from Benign Nodules

    doi: 10.1093/gpbjnl/qzae085

    Figure Lengend Snippet: Profiling of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control in the HuProt TM screening The heatmap displays a distinct distribution of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control, with generally higher levels of IgM observed in Early-LUAD. All values are normalized. The plots on the top display the data on age, sex, smoking, and alcohol consumption for each group, while the bar chart on the right side shows the sensitivity of each autoantibody. Control indicates the BLD+NHC group.

    Article Snippet: The high-throughput protein microarray HuProt TM v4.0 was obtained from CDI Laboratories (Catalog No. CDIHP-004, Baltimore, MD).

    Techniques: Control

    Verification of autoantibodies by focused microarray A . Repeated detection of pooled samples displayed high reproducibility, with an averaged correlation coefficient of 0.95. Pooled samples comprised randomly selected samples from the Early-LUAD, BLD, and NHC groups (10 samples in each group). ***, P < 0.001 ( t -test for Pearson correlation coefficients). B . The top 10/top 15 IgG and IgM autoantibodies with the most significant elevation in Early-LUAD compared to BLD/NHC/Control are displayed. These most significant autoantibodies are ranked based on their FC on the vertical axis and sensitivity on the horizontal axis. C . A descending trend in the signal distribution of IgM autoantibodies across three representative samples from the Early-LUAD, BLD, and NHC groups, respectively. The images on the left visually depict the functionality of the focused microarray, while the 3D bar plots on the right show the distribution of normalized fluorescence intensities. D . IgG and IgM types of autoantibodies showed significantly higher levels in Early-LUAD compared to BLD. *, P < 0.05; **, P < 0.01 (Welch’s t -test). FC, fold change; NS, not significant.

    Journal: Genomics, Proteomics & Bioinformatics

    Article Title: A Novel IgG–IgM Autoantibody Panel Enhances Detection of Early-stage Lung Adenocarcinoma from Benign Nodules

    doi: 10.1093/gpbjnl/qzae085

    Figure Lengend Snippet: Verification of autoantibodies by focused microarray A . Repeated detection of pooled samples displayed high reproducibility, with an averaged correlation coefficient of 0.95. Pooled samples comprised randomly selected samples from the Early-LUAD, BLD, and NHC groups (10 samples in each group). ***, P < 0.001 ( t -test for Pearson correlation coefficients). B . The top 10/top 15 IgG and IgM autoantibodies with the most significant elevation in Early-LUAD compared to BLD/NHC/Control are displayed. These most significant autoantibodies are ranked based on their FC on the vertical axis and sensitivity on the horizontal axis. C . A descending trend in the signal distribution of IgM autoantibodies across three representative samples from the Early-LUAD, BLD, and NHC groups, respectively. The images on the left visually depict the functionality of the focused microarray, while the 3D bar plots on the right show the distribution of normalized fluorescence intensities. D . IgG and IgM types of autoantibodies showed significantly higher levels in Early-LUAD compared to BLD. *, P < 0.05; **, P < 0.01 (Welch’s t -test). FC, fold change; NS, not significant.

    Article Snippet: The high-throughput protein microarray HuProt TM v4.0 was obtained from CDI Laboratories (Catalog No. CDIHP-004, Baltimore, MD).

    Techniques: Microarray, Control, Fluorescence